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| Registros recuperados: 109 | |
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| Chretiennot-dinet, Marie-josèphe; Vaulot, Daniel; Galois, Robert; Spano, Anna-maria; Robert, Rene. |
| The ingestion of 8 algal species by oyster larvae (Crassostrea gigas Thunberg, 1793) was measured by flow cytometry (FCM). In a preliminary experiment, cell size (estimated by light scatter) and chlorophyll fluorescence of 30 algal species were evaluated to select those species which could be mixed together and still be easily discriminated by FCM. Grazing experiments were carried out over 48 h with 6 and 15-day old larvae fed on 3 algal mixtures, each containing 3 different algal species. The concentration of each species was estimated at 0, 6, 12, 24 and 48 h by FCM. Grazing pressure on a given algal species was dependent upon the age of the larvae, the time of the day and the composition of the mixture. Grazing rates of older larvae were about twice... |
| Tipo: Text |
Palavras-chave: Oyster larvae; Crassostrea gigas; Grazing; Algae; Selective feeding; Flow cytometry. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00090/20164/17817.pdf |
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| Alireza,Khabiri; Mohsen,Abolhassani; Sudabeh,Aslani; Hiedeh,Darabi; Farideh,Bagheri; Mohammad-Hossein,Alimohammadian. |
| In this report we have analysed the peripheral blood lymphocyte of several patients with chronic hepatitis B virus infection with flow cytometry. Based on the presence and absence of the HBeAb, patients were divided into two groups. In both, all the patients were HBsAg positive with normal range of serum alanine aminotranferase (23.9 ± 17.8). We have found that the immunophenotypic profiles of patients were different from healthy donors with significant decrease in CD3+ T cells, specially CD8+ T cells and a siginificant increase in the CD19+ B cells. The differences were seen in other subset of T cells (CD4+) or NK cells (CD56+/CD16+ ) and HLA-DR markers were not significant. When the phenotypic profiles of both groups were compared with each other, such... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B virus; Chronic HBV infection; HBsAg; HBeAg; HBeAb; Lymphocyte subset; Flow cytometry. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000600009 |
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| Rego,E.M.; Garcia,A.B.; Carneiro,J.J.; Falcão,R.P.. |
| The distinction between normal and leukemic bone marrow (BM) B-precursors is essential for the diagnosis and treatment monitoring of acute lymphoblastic leukemia (ALL). In order to evaluate the potential use of quantitative fluorescence cytometry (QFC) for this distinction, we studied 21 normal individuals and 40 patients with CD10+ ALL. We characterized the age-related changes of the CD10, CD19, TdT, CD34 and CD79a densities in normal and leukemic BM. Compared to normal adults, the B-precursors from normal children expressed significantly lower values of CD34-specific antibody binding capacity (SABC) (median value of 86.6 vs 160.2 arbitrary units (a.u.) in children and adults, respectively). No significant age-related difference was observed in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute lymphoblastic leukemia; Lymphocytes; Quantitative fluorescence cytometry; Flow cytometry; Age-related changes. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200005 |
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| Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C. |
| Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity... |
| Tipo: Text |
Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf |
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| Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry. |
| As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater... |
| Tipo: Text |
Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf |
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| Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie. |
| in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the... |
| Tipo: Text |
Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf |
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| Escandón,Alejandro S; Miyajima,Ikuo; Alderete,Marisol; Hagiwara,Juan Carlos; Facciuto,Gabriela; Mata,Diego; Soto,Silvina M. |
| The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant.... |
| Tipo: Journal article |
Palavras-chave: Chimera; Flow cytometry; Micropropagation; Ornamental plants; Tetraploid. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200010 |
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| Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek. |
| Using flow cytometry, 234 Macoma balthica were examined during a survey to determine frequency of neoplasia in the Gulf of Gdansk (Poland). Clams were collected in 4 locations and DNA content in gill tissue cells was determined by flow cytometry using propidium iodide staining. Cell permeabilization was induced by osmotic shock. Prevalence of neoplasia ranged from 9.6 to 26.7 % depending on location. DNA content in aneuploid cells was higher than in normal dividing cells. The fluorescence value for aneuploid cells corresponded to tetraploid/pentaploid cells. Three stages of neoplasia were defined, based on the percentage of aneuploid cells determined by flow cytometry. Histopathological and cytogenetic analyses were also carried out on the same clams for... |
| Tipo: Text |
Palavras-chave: Prevalence; Macoma balthica; Flow cytometry; Gill neoplasia. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2919.pdf |
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| Registros recuperados: 109 | |
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